22K 



Gelatin liquefied. Ps. OCHRACEA GROUP 

 Gelatin not liquefied. Ps. TURCOSA GROUP 

 ft Pigment blue-violet. 



Gelatin liquefied. Ps. JANTHINA GROUP. 

 Gelatin not liquefied. Ps. BEROUNENSIS GROUP. 

 Produce a greenish-bluish fluorescence in culture media 

 f Gelatin liquefied. Ps. PYOCYANEA GROUP. 

 ft Gelatin not liquefied. Ps. SYNCYANEA GROUP. 

 2. With endospores, aerobic and facultative anaerobic. 



a. Non-chromogenic. 



* Rods not swollen at sporulation. Ps. ROSEA GROUP. 



: * Rods swollen at one end at sporulation. Ps. TROM- 



MELSCHLAGER GROUP. 



b. Produce a greenish-bluish fluorescence in culture media. 



* Gelatin liquefied. Ps. VIRIDESCENS GROUP. 

 N * Gelatin not liquefied. Ps. UNDULATA GROUP. 



B. Do not grow in nutrient gelatin or other organic media. Ni- 



TRIMONAS GROUP. 

 II. Cell plasma with a reddish tint, also with sulphur granules. CHRO- 



MATIUM GROUP. 



MICROSPIRA (Migula). 

 I. Cultures show a bluish-silvery phosphorescence. PHOSPHORESCENT 



GROUP. 



II. Cultures not phosphorescent. 

 A. Gelatin liquefied. 



1. Cultures show the nitro-indol reaction. 



a. Very pathogenic to pigeons. MSP. METSCHNIKOVI 



GROUP. 



b. Not distinctly pathogenic to pigeons. CHOLERA GROUP. 



2. Nitro-indol reaction negative or very weak, at least after 



twenty-four hours. CHOLERA NOSTRAS GROUP. 

 B: Gelatin not liquefied or only slightly so. MSP. SAPROPHILA 

 GROUP. 



MYCOBACTERIUM (Lehmann-Neumann). 



I. Stain with basic anilin dyes, and easily decolorized by mineral 

 acids when stained with carbol-fuchsin. 



A. Grow well on nutrient gelatin. Gelatin liquefied very slowly 



or merely softened. 



1. Stain by Gram's method. SWINE ERYSIPELAS GROUP. 



2. Not stained by Gram's method. GLANDERS GROUP. 



B. Little or no growth in ordinary nutrient gelatin. 



1. Grow well in nutrient bouillon at body temperatures. 



a. Stained by Gram's method. Rods cuneate clavate 

 irregularly swollen. DIPHTHERIA GROUP. 



2. No growth in nutrient bouillon or on ordinary culture media. 



Rods slender, tubercle-like. 



a. Stain by Gram's method. LEPROSY GROUP. 



b. Do not stain by Gram's method. INFLUENZA GROUP. 



3. No growth in nutrient bouillon or on ordinary culture 



media. Rods variable. ROOT-TUBERCLE GROUP. 

 II. Not stained with aqueous solutions of basic anilin dyes ; not easily 

 decolorized by acids. TUBERCLE GROUP. 



COCCACEJE. 



Cells in their free condition globular, becoming slightly elongated 

 before division. Cell division in one, two, or three directions 

 of space. 



