Cultivation 



369 



anaerobic organism, and can be grown in bouillon, upon 

 agar-agar, better upon glycerin agar-agar, very well upon 

 blood-serum, and quite characteristically upon potato. 

 Gelatin is not liquefied by the glanders bacillus. 



Colonies. Upon 4 per cent, glycerin agar-agar plates 

 the colonies appear upon the second day as whitish or pale 

 yellow, shining round dots. Under the microscope they 

 are brownish-yellow, thick, and granular, with sharp borders. 



Bouillon. In broth cultures the glanders bacillus causes 

 turbidity, the surface of the culture being covered by a 

 slimy scum. 



Agar-agar. Upon agar-agar and glycerin agar-agar the 

 growth occurs as a moist, shining layer. 



Fig. 107. Culture of glanders bacillus upon cooked potato (Loffler). 



Blood-serum. Upon blood-serum the growth is rather 

 characteristic, the colonies along the line of inoculation 

 appearing as circumscribed, clear, transparent drops, which 

 later become confluent and form a transparent layer un- 

 accompanied by liquefaction. 



Potato. The most characteristic growth is upon potato. 

 It first appears in about forty-eight hours as a transparent, 

 honey-like, yellowish layer, developing only at incubation 

 temperatures, and soon becoming reddish-brown in color. 

 As this brown color of the colony develops, the potato for a 

 considerable distance around it becomes greenish-brown. 



