470 Vibrio Schuylkiliensis 



Gelatin Punctures. In gelatin puncture cultures the 

 appearance is exactly like the true cholera spirillum. At 

 times the growth is a little more rapid. 



Agar-agar. The growth on agar is luxuriant, and gives 

 off a pronounced odor of indol. 



Blood-serum. Loffler's blood-serum is apparently not 

 a perfectly adapted medium, but upon it the organisms 

 grow, with resulting liquefaction. 



Potato. Upon potato, at the point of inoculation a thin, 

 glazed, more or less dirty yellow growth, shading to brown 

 and sometimes surrounded by a flat, dry, lusterless zone, is 

 formed. 



Milk. In litmus milk a reddish tinge develops after 

 the milk is kept twenty-four hours at body-temperature. 

 After forty-eight hours this color is increased and the milk 

 coagulates. 



Metabolic Products. In peptone solutions indol is 

 easily detected. No gas is produced in glucose-containing 

 culture media. Acids and coagulating enzymes are formed. 

 The organism is a facultative anaerobe. 



Vital Resistance. The thermal death-point is 50 C. 

 maintained for five minutes. 



Pathogenesis. The organism is pathogenic for pigeons, 

 guinea-pigs, and mice, behaving much like Spirillum 

 metschnikovi. No Pfeiffer's phenomenon was observed 

 with the use of serum from immunized animals. 



Immunity. Immunity could be produced in pigeons, 

 and it was found that the serum was protective against both 

 Vibrio Schuylkiliensis and Spirillum metschnikovi, the im- 

 munity thus produced being of about ten days' duration. 



In a second paper by Abbott and Bergey * it was shown 

 that the vibrios occurred in the water during all four seasons 

 of the year, and in all parts of the river within the city, both 

 at low and at high tide. They were also found in the 

 sewage emptying into the river, and in the water of the 

 Delaware River as frequently as in that of the Schuylkill. 



One hundred and ten pure cultures were isolated from 

 the sources mentioned and subjected to routine tests. It 

 was found that few or none of them were identical in all 

 points. There seems to be, therefore, a family of river 

 spirilla, closely related to one another, like the different 

 colon bacilli. 



* "Journal of Experimental Medicine," vol. n, No. 5, p. 535. 



