i62 PATHOGENIC BACTERIA. 



The animal should be tacked to a board if small, or 

 tied, by cords fastened to the legs, to the corners of a 

 table if large, and should be dissected with sterile knives 

 and scissors. When a culture is to be made from the 

 interior of an organ say the spleen it should be incised 

 deeply with a sterile knife and the culture made from 

 its centre. 



Fragments intended for subsequent microscopical ex- 

 amination should be cut very small (cubes of i c.cm.) r 

 placed in absolute alcohol for a few hours, then trans- 

 ferred to weaker alcohol, 80-90 per cent., for preserva- 

 tion. The technique of imbedding and staining the tis- 

 sues can be found in almost any reliable text-book on 

 pathology or on the special subject of microscopical 

 technique. 



