244 PATHOGENIC BACTERIA. 



lander and a number of colonies consisting of fine, slender, 

 often somewhat nodose bacilli about the size and form of 

 the lepra bacillus. 



These colonies were grayish-yellow, humped in the 

 middle, 1-2 mm. in diameter, irregularly rounded, and 

 irregular at the edges. They could be inverted entire 

 with the platinum wire and were excavated on the under 

 side. The consistence was crumbly. 



When a transfer was made from one of these colonies 

 to fresh media, in a few days the growth became apparent 

 and assumed a band-like form, with a plateau-like eleva- 

 tion in the center. 



The bacillus thus isolated grew with moderate rapidity 

 upon all the ordinary culture-media except potato. Upon 

 blood-serum the growth was more luxuriant and fluid 

 than upon the solid media. Upon coagulated serum the 

 growth was rather dry and elevated, and was frequently 

 so loosely attached to the surface of the medium as to 

 be readily lifted up by the platinum wire. 



The growth was especially good upon sheep's blood- 

 serum witli the addition of 5 per cent, of glycerin. The 

 growth upon the L,6ffler-mixture was excellent. 



Upon agar-agar the growth is not so good as upon 

 blood-serum ; it is more luxuriant iipon glycerin agar- 

 agar than upon plain agar-agar; it is grayish and flatter 

 upon agar-agar than upon blood-serum. The growth 

 never extends to the water of condensation to form a 

 floating layer, as does that of the tubercle bacillus. 



The colonies that form upon agar-agar are much like 

 those described by Bordoni-Uffredozzi, and appear as iso- 

 lated, grayish, rounded flakes, thicker in the center than 

 at the edges, and characterized by an irregular serrated 

 border from which a fine irregular network extends upon 

 the medium. These projections consist of bundles of the 

 bacilli. 



Upon gelatin the bacillus develops well after it has 

 grown artificially for a number of generations. Upon 

 the surface of gelatin the growth is, in general, similar 



