GLANDERS. 251 



four days the disease becomes established. The testicles 

 enlarge a little ; the skin over them becomes red and 

 shining. The testicles themselves begin to suppurate, 

 and often discharge through the skin. The animal dies 

 in about two weeks. If such an animal be killed and its 

 testicles examined, the tunica vaginalis testis will be 

 found to contain pus, and sometimes to be partially ob- 

 literated by inflammatory exudation. The bacilli are pres- 

 ent in this pus, and can be secured from it in pure cultures. 



The value of Strauss' s method has, however, been less- 

 ened by the discovery by Kutcher, 1 that a new bacillus, 

 which he has classified among the pseudo-tubercle ba- 

 cilli, produces a similiar testicular swelling when injected 

 into the abdominal cavity. 



The purulent discharges from the noses of horses 

 and from other lesions of large animals generally con- 

 tain very few bacilli, so that their detection by the 

 use of the guinea-pig inoculation is made much more 

 simple. 



The bacillus is an aerobic organism, and can be grown 

 in bouillon, upon agar-agar, better upon glycerin agar- 

 agar, very well upon blood-serum, and quite character- 

 istically upon potato. It grows in gelatin, but this is 

 not an appropriate medium, because the bacillus develops 

 best at temperatures at which the gelatin is liquid. 



Upon 4 per cent, glycerin agar-agar plates the colonies 

 appear upon the second day as pale-yellow or whitish, 

 shining round dots. Under the microscope they appear 

 as brownish-yellow, thick granular masses with sharp 

 borders. 



The culture upon agar-agar and glycerin agar-agar 

 occurs as a moist, shining layer not possessed of distinct 

 peculiarities. Upon blood-serum the growth is rather 

 characteristic. The colonies along the line of inoculation 

 first develop as circumscribed, clear, transparent drops, 

 which later become confluent and form a transparent 

 layer unaccompanied by liquefaction. 



1 Zeitschrift fur Hygiene, Bd. xxi., Heft i., Dec. 6, 1895. 



