436 PA THOGENIC BA CTERIA . 



six days the islands are still more compact and solidified. 

 If the vessel be disturbed, the islands fall like snow and 

 are deposited at the bottom. 



Upon gelatin plates at 22 C. the colonies may be ob- 

 served in twenty-four hours by the naked eye. They are 

 pure white or yellowish-white, spherical in the deep gela- 

 tin, flat upon the surface, and are about the size of a 

 pin's head. The gelatin is not liquefied. The borders 

 of the colonies are, upon microscopic examination, found 

 to be sharply defined and to become more granular as 

 their age increases. The superficial colonies occasionally 

 are surrounded by a fine, semi-transparent zone. 



In gelatin puncture-cultures the development is scant. 

 The medium is not liquefied (?); the growth takes place 

 in the form of a fine duct, little points being seen on the 

 surface and in the line of puncture. 



Upon agar-agar glycerin agar-agar is best the bacilli 

 grow freely, the colonies being whitish in color, with a 

 bluish tint by reflected light. Under the microscope 

 they appear moist, with rounded, uneven edges. The 

 small colonies are said to resemble little tufts of glass- 

 wool; the larger ones have large round centers. Micro- 

 scopic examination of the bacilli grown upon agar-agar 

 reveals the presence of long chains resembling strepto- 

 cocci. 



Klein 1 states that the colonies develop quite readily 

 upon gelatin made from beef-bouillon (not infusion), 

 appearing in twenty-four hours, at 20 C., as small, gray, 

 irregularly rounded dots. Magnification shows the col- 

 onies to be serrated at the edges and made up of short, 

 oval, sometimes double bacilli. Some colonies contrast 

 markedly with their neighbors in that they are large, 

 round, or oval, and consist of longer or shorter, straight 

 or looped threads of bacilli. The appearance was much 

 like that of the young colonies of the Proteus vulgaris. 

 At first Klein regarded these as contaminations, but later 

 he was led to believe that their occurrence was character- 



1 Ccntralbl.f. Bakt. u. Pamsittnk., xxi., Nos. 24 and 25, July 10, 1897. 



