470 APPENDIX. 



dilute solution of the globulin, (c) on diluting 20 times with water 

 after acidifying faintly with acetic acid (page 331). Note the coag- 

 ulation-point of the serum-globulin in the presence of 5 per cent, 

 of sodium chloride as described below. 



B. Note the solubility of the serum-albumin in water ; estimate 

 the coagulation-point in the presence of 5 per cent, of sodium 

 chloride. To this end, a test-tube is filled about one-half with the 

 solution and immersed in a large beaker with water ; a thermometer 

 registering 100 C. should dip nearly to the bottom of the test-tube ; 

 heat slowly. 



7. Remove all albumins from 20 to 30 c.c. of serum, according 

 to Cavazzani's method (page 333). In the resulting solution de- 

 monstrate the presence of glucose by means of the phenyl-hydrazin 

 test. 



Exercise XLI. 1. Examine fresh blood directly with a spectro- 

 scope. 



2. Estimate the amount of haemoglobin in human blood with 

 Dare's hsemoglobinometer, or with v. FleischPs haBmometer. 



3. Prepare some crystalline oxyhaemoglobin from dog's blood 

 (100 c.c.) (page 350) ; examine the resulting product spectroscop- 

 ically ; note the change in the spectrum on the addition of caustic 

 alkali and reduction with ammonium sulphide (page 346). 



4. Prepare some haemin from oxyhsemoglobin and from this, in 

 turn, hsematin (page 347) ; note the spectrum of the latter in acid 

 and in alkaline solution. 



5. Perform the hsemin test with a drop of blood. To this end, 

 let a delicate film of sodium chloride form in the middle of a slide ; 

 place on this a drop of blood ; let dry and add glacial acetic acid, a 

 drop at a time, while heating very gently over a flame ; examine 

 from time to time with a microscope. Finally add a drop of 

 glycerin, cover, and examine. Note the color, form, and varying 

 size of the crystals. 



6. Prepare some carbon monoxide haemoglobin from oxyhsemo- 

 globin (page 352). Note the spectrum. 



7. Prepare a solution of ha3matoporphyrin in a test-tube by 

 adding about 5 drops of blood, drop by drop, and shaking con- 

 stantly, to 8 or 10 c.c. of concentrated sulphuric acid ; examine with 

 a spectroscope (page 355). 



Exercise XLII. 1. Hash 100 grammes of lean muscle-tissue 

 (beef) ; suspend in 300 c.c. of water ; stir well ; allow to stand for 

 a couple of hours ; filter through muslin and then through filter- 

 paper, (a) Examine the reaction of the filtrate. (6) Demonstrate 

 the presence of three albumin fractions by fractional coagulation. 

 (Arrange the apparatus as described above, XL., 6, B.) Heat very 

 slowly and filter whenever one fraction has been coagulated, (c) 

 Prepare myosin from the meat residue after thorough washing with 

 water as follows : prepare a 15 per cent, solution of ammonium 

 chloride, suspend the meat in this ; stir well and allow to stand for 



