82 THE CHEMISTRY OF THE TISSUES AND ORGANS. 
viscidity, and it was the absence of this character which led me to the 
erroneous conclusion that no nucleo-proteid had gone into solution. 
The sodium sulphate extract contains two proteids, one which co- 
agulates at 48°-50° C., the other at 75° C. The first, which I called cell 
globulin-a, is really a globulin ; it yields no nuclein on gastric digestion ; 1 
but the second, which I called cell globulin-/?, though like a globulin in its 
solubilities, is really the same nucleo-proteid which by treatment with 
other salts is rendered viscid. 2 That this substance is related to, if not 
identical with, the fibrin ferment or its zymogen (Pekelharing) has been 
rendered probable by the researches of Pekelharing and myself. 
The albumin is only present in minute quantities ; its properties are 
like those of serum albumin, and it may partly arise from blood or 
lymph imperfectly washed away from the cells. 
Proteoses and peptone, when present, are the result of post-mortem 
changes, or of manipulations during the processes employed in separating 
the other proteids. 
Myosin is absent. 
Lilienf eld :; has carried out a similar research on the chemistry of 
cells which he obtained from the thymus, by the usual means of pressure 
and the centrifuge. He found a proteid corresponding to cell globulin-a 
coagulating at 48° C, and another corresponding to cell albumin 
coagulating at 73-75° C. The nucleo-proteid which he obtained by my 
sodium chloride process contained C, 5346 ; H, 7*64 ; N, 15 - 57, and P, 
0*433 per cent. The alcoholic extract of the cells contained protagon, 
amido-valeric acid, inosite, and monopotassium phosphate. 
By Wooldridge's method he obtained the nucleo-proteid he has 
called " nucleo-histon " (see p. 68), and he considers that this, in part at 
any rate, is derived from the nuclei. Its percentage composition is C, 
48-46 ; H, 7"0 ; N, 16-86 ; P, 3-025 ; and S, 0-701. The action of artificial 
gastric juice, or of - 8 per cent, hydrochloric acid, on this, is to separate 
the nuclein from the proteid, which goes into solution as peptone. The 
nuclein contains 4 - 991 per cent., and the nucleic acid prepared from 
this 9 - 94 per cent., of phosphorus. 
In the following table he gives the quantitative composition of 
leucocytes : — 
Water 88-51 
Solids 11-49 
One hundred parts of the solids contain — 
Total phosphorus . . . . . . 3 -01 
Total nitrogen ....... 15 -03 
Proteid 1'76 
Nuclein ........ 68 - 78 
Histon {i.e. proteid part of the nucleo-proteid) . 8 '67 
Lecithin 7'51 
Fat 4-02 
Cholesterin 4 40 
Glycogen . . . . . . . .0-80 
1 Halliburton, Journ. Physiol., Cambridge and London, 1892, vol. xiii. p. 806. 
2 Ibid., 1895, vol. xviii. p. 312. Pekelharing showed this also to be the case. 
3 Ztschr. f. -physiol. Chem., Strassburg, Bd, xviii. S. 473. 
