86 THE CHEMISTRY OF THE TISSUES AND ORGANS. 
nuclein on gastric digestion ; (3) a globulin coagulating at 75° C. ; and 
(4) alkali albumin. 
A good many years later, 1 I repeated these experiments ; and, like 
Plosz, failed to find myosin. Myosin appears to be a specific constituent 
of muscle, and has not been found anywhere else. The hardening that 
occurs in the liver after death, and which is very slight, is possibly due 
to the solidification of the fat in the cells ; though it is also quite possible, 
as Plosz suggests, that if coagulation does occur in the cells with the 
formation of a myosin-like clot, this takes place so rapidly that our 
present methods do not enable us to separate its precursor from the cells. 
The proteids I obtained by the use of saline solutions were four in 
number : — 
1. A globulin (cell-globulin) coagulating at 45°-50° C. 
2. A nucleo-proteid which coagulates at about 60° C, and is identical 
with that obtainable by Wooldridge's acetic acid method from the cells. 
It contains P45 per cent, of phosphorus. It does not become viscous on 
admixture with neutral salts, and the sodium chloride method of 
preparing nucleo-proteicls is not applicable to it. It produces intra- 
vascular coagulation. 
3. A globulin coagulating at 70° C. 
4. An albumin in mere traces, which coagulates at about the same 
temperature. 
Other organic constituents of the liver cells. — Urea, uric acid (especially 
in birds), xanthine, and hypoxanthine, are found in the liver ; 2 leucine 
and tyrosine are found in cases of acute yellow atrophy, and in 
phosphorus poisoning. 3 Various other substances have been described 
as occasional constituents. 4 
A substance called jecorin, containing phosphorus (C K , : JT 1SG N 5 SP.;0_ 1 -), 
was separated from the liver by Drechsel. 6 In its properties it some- 
what resembles lecithin : it, however, reduces Fehling's solution, which 
lecithin does not. According to Baldi, 6 it occurs in many other organs — 
spleen, muscle, brain, etc. 
The question of the iron-containing nucleins of the liver (Zaleski's 
hepatin, Schmiedeberg's ferratin, 7 etc.) is alluded to on p. 69. The 
iron in the liver is increased in diseases, like pernicious anaemia, which 
lead to increased destruction of red blood corpuscles ; it is normally 
greater in young (especially new-born) animals than in older ones. 
Animals appear to enter the world with a store of iron in the liver, and 
to a less degree in the spleen, which lasts them until they are able to 
take foods other than milk, which is poor in iron. 8 
1 journ. Physiol., Cambridge and London, 1S92, vol. xiii. p. 806. 
'- Scherer, Ann. d. Chem., Leipzig, Bd. cvii. S. 314 ; Cloetta, ibid., Bd. xevii. S. 289. 
8 Sotnitschewsky, Ztschr. f. physiol. Chem., Strassburg, Bd. iii. S. 391; see also 
Rohmann, Berl. klin. Wchnschr., 1888, S. 43 aud 44. 
4 Guanine, inosite, scyllite (Frerichs and Stadeler, Mitth. d. Zurich, natwr. Gfesellsch. 
1858) ; cystine (Hoppe-Seyler, " Physiol. Chem.," S. 718) ; sarcolactic acid, probably formed 
after death. 
5 Journ. f. prakt. Chem., Leipzig, Bd. xxxiii. S. 435. 
B Arch./. Physiol., Leipzig, 1887, Suppl., S. 100. 
7 For recent work in ferratin and iron in the liver, and absorption of iron compounds 
as food, see F. Vav, Ztschr. f. •physiol. Chem., Strassburg. Bd. xx. S. 377: Woltering, 
ibid., Bd. xxi. S. "l86 ; Hall, Arch./. Physiol., Leipzig, 1896, S. 49, 142: Cloetta, 
Arch. f. exper. Path. u. Pharmakol., Leipzig, 1896, Bd. xxxiii. S. 6; Hochhaus and 
Quincke, ibid., S. 152 ; Quincke, ibid., S. 182. 
8 Meyer and Pernou, Ztschr. f. Biol., Munchen, Bd. xxvii. S. 439; Lapicque, Compt. 
rend. Soc. de, biol., Paris, tome xli. p. 435. 
