i6 



the swollen microbe assumes an oblong, cylindrical 

 or dumb-bell form, and then by partition it is divided 

 into two, three, or more segments, and finally develops 

 into a kind of hyphae ; secondary hyphae appear in 

 the course of time also by budding, and afterwards 

 tertiary hyphae appear from these. 



Plate ! shows the organism as demonstrated by 

 Tokishige from the so-called Japanese farcy. 



Plate II shows the organism from a preparation 

 prepared and stained by Professor Mettam, of the 

 Royal Veterinary College of Ireland. 



Plate III shows a pustule in varying stages, and 

 also the lesions in the subcutaneous tissues, from a 

 photo by Tokishige. 



Culture 



This is obtained with difficulty. The growth is 

 always slow, and develops much better in an acid than 

 alkaline medium, the temperature not appearing to have 

 any marked effect. Tokishige obtained a growth in 

 peptonized bouillon, agar-agar, nutrient gelatine, and 

 on potato. 



In bouillon, after seventeen days, a white flaky 

 deposit, which includes hyphae and cells, makes its 

 appearance. On agar, after thirty days, the vegetation 

 first becomes apparent in the form of greyish-white 

 grains, and in from forty to fifty days a single colony 

 attains a diameter of i to 4 mm., and becomes distinctly 

 prominent over the surface of the medium. In a full 

 grown colony the surface is wrinkled, the colony is very 

 dense and difficult to dissect with a platinum wire or 

 crush under a cover-glass. 



Microscopically, it consists of conglomerated 

 masses, composed of hyphae, spherical bodies, and a 



