I? 



number of free granules. The addition of grape sugar 

 or glycerine to the medium has no influence on the 

 vegetation. 



On nutrient gelatine the vegetation takes place 

 more on the upper strata, and in fifty-six days some 

 yellowish-white sandy masses of an irregular shape, 

 measuring i to 4 mm. in diameter, make their appear- 

 ance. The gelatine does not liquify except by heating, 

 when the colony sinks and its growth stops. 



On potato the growth is more rapid, and the 

 colonies are of a light-brown colour, but otherwise 

 they resemble the growths obtained on agar-agar. 



Fluid media may also be used for the cultivation 

 of this organism, but peptone must be added. Tokishige 

 states that it neither grows on an infusion of horse 

 dung or hay, nor in a solution of sugar. 



Marcone cultivated the cryptococcus on horse 

 serum mixed with 2 per cent, solution of agar-agar, 

 glycerine and cane sugar, and got after fifteen days, at 

 a temperature of from 2'^°~37° centigrade, some fine 

 greyish specks making their appearance and slowly 

 enlarging at several points of the medium ; the growth 

 then appears to cease and falls to the bottom of the 

 flask, where it forms a thick uniform, white deposit. 



When the culture is old, if the flask be shaken, it 

 becomes opaque, and does not clear up for some con- 

 siderable time. 



As might be anticipated, the cryptococci or sac- 

 charomyces are often associated with staphylococci, 

 diplococci or streptococci, and other organisms, which 

 are especially met with in the contents of the pustules 

 and abscesses and in the lymphatic glands. 



Plate IV shows a culture made by Tokishige. 



