35 



Experimental inoculation into the peritoneal cavity 

 of the guinea-pig produces orchitis in six to eight 

 days, similar to that obtained in glanders and, tor 

 that reason, may lead to confusion ; however, the 

 question is easily solved by resorting to the mallein 

 test, and also to the use of other experimental animals, 

 e.g., donkeys. The inoculated guinea-pig very seldom 

 succumbs to the disease, but it is not uncommon for 

 one of the testicles to be almost entirely destroyed ; in 

 ordinary subcutaneous inoculation a large abscess forms 

 in four or five days, and the adjacent lymphatics become 

 invaded. 



Experimental subcutaneous inoculation of a horse, 

 mule, or donkey produces an abscess in six to ten 

 days. 



Inoculation with a culture of the organism kills a 

 guinea-pig in twenty-four to forty-eight hours. 



Rabbits resist an intraperitoneal injection of a 

 culture. 



White mice are killed in twenty-four to forty- 

 eight hours, and a culture of the bacillus can be generally 

 obtained from the blood. 



Pigeons sometimes die four to six days after an 

 intravenous inoculation, but fowls are refractory. 



The organism has very little resistance and is 

 destroyed at a temperature of 56'^C. in one hour, and at 

 65°C. in less than a quarter of an hour. And, contrary 

 to the epizootic form, the treatment is simple — the 

 disease is less indolent — and if experimental inoculation 

 and mallein testing is resorted to there should be no 

 difficulty in the diagnosis. 



3. Simple lymphangitis and its sequelae, i.e., suppura- 

 tive lymphangitis^ due to acute inflammation and septic 

 infection, is differentiated from epizootic lymphangitis by 

 the absence of the characteristic symptoms and of the 



