39 



the results show that infection is really rather difficult, 

 and may at times fail to reproduce the disease even in 

 susceptible animals, or it may only show itself after a long 

 time. 



In my experiments carried out in India, the first 

 symptom observed, after a subcutaneous inoculation 

 on the sideof a donkey's neck (simultaneous inoculations 

 having been also made in both nostrils by scarification), 

 was a small, round, hard, slightly painful enlargement 

 about the size of a hazel nut at the seat of inoculation on 

 the neck after thirty-two days ; this remained much the 

 same for about a week, except that the painfulness 

 gradually subsided, and there was a slight inchnation for 

 the swelling to decrease. 



On the forty-fifth day the enlargement increased 

 to three times its previous size, and it became softer 

 and more painful. On the forty-eighth day it returned 

 to its original size. On the fifty-third day a small 

 vesicle was noticed to have broken out on the edge of the 

 near nostril, leaving a minute ulcer, on a scar resulting 

 from scarification, just where the skin adjoins the nasal 

 mucous membrane. On the fifty-fourth day the ulcer on 

 edge of near nostril had increased in size, and was dis- 

 charging slightly. Two small ulcers had now developed 

 at the edge of the off nostril, one of which was almost 

 on the mucous membrane itself. At the same time the 

 enlargement at the seat of inoculation on the neck had 

 become partially divided into two small nodules, one of 

 which was softening and preparing to form into a 

 pustule. 



On the fifty-eighth day a small chain of vesicles 

 had broken out along the scar on the edge of the near 

 nostril, and a pustule had now formed at the site of 

 inoculation on the neck. 



On the sixty-seventh day the enlargement on the near 

 side of neck had increased, the pustule had burst, and 



