BACTERIUM PULLORUM INFECTION IN FOWL. 



65 



clinically white diarrhoea, and tliese conformed morphologically, bio- 

 chemically and serologically to this group of organisms. It was further 

 decided to study the uniformity of these 112 cultures biochemically and 

 serologically, and to determine how many of them gave reactions which 

 were similar to the reactions of its close relative, the fowl typhoid organism 

 {Bad. sanguvnarium). The cultures of Bad. sanguinarium were isolated 

 from birds sent here for diagnosis, and the Smith, Cornell and Gage 

 strains. There were five strains in this list. The two other than the 

 three mentioned appeared typical of sanguinarium, were isolated during 

 the early part of 1920, and designated the Humphrey and Massachusetts 

 strains, respectively. 



The following table lists the cultures of Bad. pullorum isolated and 

 studied during the course of this work: — 



Table 1. — Strains of Baderhnn Pullorum studied in this Investigation. 



Bacterium Pullorum. 



Source of Culture. 



When Isolated 

 and Studied. 



Strain No. 1 . 



Strain No. 2 . 



Strain No. 3 . 



Strain No. 4 . 



Strain No. 5 . 



Strain No. 6 . 



Strain No. 7 . 



Strain No. 8 . 



Strain No. 9 . 



Strain No. 10 . 



Strain No. 11 . 



Strain No. 12 . 



Strain No. 13 . 



Strain No. 14 . 



Strain No. 15 . 



Strain No. 16 . 



Strain No. 17 . 



Strain No. 18 . 

 Strain No. 19 . 



M. A. C. Amherst, Mass. Isolated March, 1914, 



from M. A. C. chick. Used in summer of 1914 as 



Strain A. 

 Experimental material from this laboratory. From 



unabsorbed yolk of chick inoculated summer of 



1913 with Ss (Ss from Cutler egg). Used in 



summer of 1914 as Strain B. 

 Isolated from material sent to laboratory. Used as 



Strain C in summer of 1914. 

 Bridgewater, Mass. Isolated from Cutler chick. 



Used as So in 1913. Used as Strain D in 1914. 

 Maryland. Used at Maryland Experiment Station 



in 1911. 

 Sterling, Mass. Isolated 1914. Trask Strain. Used 



as Strain F in summer of 1914. 

 Holliston, Mass. Isolated from chicks sent by C. E. 



Cristman, Silverwood Farm, Holliston, Mass. 



These chicks were bought of A. B. H. Arnold, 



Holliston, Mass. 

 M. A. C. Amherst, Mass. No. 231 (2703) from un- 

 absorbed yolk (chick). 

 Holliston, Mass. Isolated from unabsorbed yolk of 



chick. Isolated from liver of chick. 

 Northborough, Mass. Isolated from liver of chick . 



Franklin, Mass. 11-1 isolated from unabsorbed 



yolk of chick No. 2; 11-2 isolated from liver of 



chick No. 5. 

 North Hadley, Mass. 12-1 from unabsorbed yolk 



of chick No. 1; 12-2 from unabsorbed yolk of 



chick No. 4; 12-3 from unabsorbed yolk of chick 



No. 9. 

 Kingston, Mass. Isolated from unabsorbed yolk 



of chick No. 2. 

 Center Marshfield, Mass. Isolated from unabsorbed 



yolk of chick No. 4. 

 Brookline, Mass. Isolated from unabsorbed yolk 



of chick No. 1. 

 Amherst, Mass. Isolated from liver of chick No. 1 ; 



16-2 isolated from unabsorbed yolk of chick No. 1 ; 



16-3 isolated from liver of chick No. 2. 

 Southborough, Mass. 17-1 isolated from liver of 



chick No. 1; 17-2 isolated from heart of chick 



No. 2; 17-3 isolated from heart of chick No. 3; 



17-4 isolated from unabsorbed yolk of chick No. 



4; 17-5 Isolated from unabsorbed yolk of chick 



No. 5; 17-6 isolated from unabsorbed yolk of 



chick No. 6. 

 Littleton, Mass. 18-1 isolated from heart of chick 



No. 1; 18-2 isolated from liver of chick No. 1. 

 Andover, Mass. Isolated from unabsorbed yolk of 



chick No. 1. 



March, 1914 



May 1, 1914 

 Feb. 20, 1915 



Mar. 31, 1915 



Apr. 1, 1915 



Apr. 5, 1915 



Apr. 5, 1915 

 Apr. 6, 1915 

 Apr. 7, 1915 

 Apr. 12, 1915 



Apr. 16, 1915 



Apr. 17, 1915 

 Apr. 22, 1915 



