16 



injection was made. The injection of the tuberculin under the skin 

 was made by the use of a hypodermic syringe, at a point just back of 

 the scapula about one-third the distance from the spines of the verte- 

 brae to the point of the elbow. The quantity of tuberculin used in 

 each case will be found recorded in the tables of the tests. It varied 

 according to the size and condition of the animal and ranged from 

 0.05 c. c. to 0.5 c. c. of the concentrated drug (ten times the quantity 

 of the dilute solution). 



The syringe before being used and between injections in different 

 animals was in all cases carefully treated with 5% solution of car- 

 bolic acid or a solution of corrosive sublimate, one part of the drug 

 to 1000 parts of water. After the tuberculin had been used, inmost 

 cases, the part was again wet with the antiseptic solution. In the 

 use of tuberculin, care was taken to get the solution well under the 

 skin but not into the fleshy part beneath. 



Subsequent to the administration of the drug, during the continu- 

 ation of the test, large quantities of cold water were not allowed ; in 

 all other respects the animals were given their usual treatment. 



For a period of about twenty-four hours after injection the tem- 

 perature was taken at intervals of several hours, and the animals 

 were closely watched to determine the effects of the tuberculin. In 

 some animals there was noticed at the point of injection a somewhat 

 hard and painful swelling, in others trembling of the muscles, most 

 marked about the region of the hips, in some there was suspension 

 of rumination, in others, slight diarrhoea and lessened secretion of 

 milk. All of these effects were, however, only temporary. In the 

 autopsies of animals recently treated careful examination failed to 

 reveal any serious or permanent changes either at the point of injec- 

 tion or in other parts of the body as a result of the use of tuberculin. 

 So far as determined no particular condition of the animals predis- 

 posed to the appearance of these slight symptoms. 



In connection with the records of the first three animals tested 

 there appears a short history of the animals and after the table of 

 the temperatures etc. a record of the post mortem examination. In 

 all others the records are arranged in tabulated form. 



Jersey i>ull, Don Pedro Torment. Born January 188H. 

 Presented to the college in 18.S9. During the summer of 

 1891 he was worked in yoke with a purebred Guernsey bull 

 and was to all appearances perfectly healthy. At this time 

 the foreman on the farm said that his power of endurance 

 was greater than that of the animal with which he was worked. 



