37 



Into this there was inserted a piece of the medulla of the cow's brain 

 about the size of a small pea. The free edge of the skin was then 

 returned to its place and the parts treated with an antiseptic sohilion. 

 This wound healed firmly by the first intention. No evidence of 

 suppuration ever appeared. 



In obtaining the parts of the cow's brain for use, they were in 

 every instance cut and handled with sterilized instruments. 



These rabbits were kept in separate cages and daily supplied with 

 water, and food which consisted of a mixture of grain composed of 

 wheat bran, oats and corn, and green food of either clover or plan- 

 tain leaves. 



On the morning of June twenty-seventh rabbit No. 1 was noticed: 

 to be ailing. He refused food and was inclined to remain quiet,, 

 crouched in one corner of his cage. On the following day his con- 

 dition was even worse ; he remained nearly motionless during the 

 whole time in the corner and it was with difficulty that he could be 

 made to move. A progressive paralysis appeared in the posterior- 

 extremities and later it extended to the anteiior portions of the body. 

 This paralj'sis became so severe that the animal was unable to use 

 its legs suflflciently to move about the cage for a few hours before its 

 death. On the afternoon of June twenty-ninth he died. An autopsy 

 the next day showed that the opening in the skull had healed per- 

 fectly and that there was no alteration in the structure of the brain 

 or its coverings at the point of injection. All the organs of the body 

 were normal. 



The brain and a part of the spinal cord were removed and a smalt 

 portion of the medulla taken with sterilized instruments, broken up- 

 and mixed with distilled sterile water. At two o'clock p. m. June 

 thirtieth. No. 3 was selected and after clipping the hair from the 

 skin above the right eye and cleansing it with an antiseptic solution, 

 an opening was made into the cranial cavity as in the case with No. 

 1. Then about 0.1 c. c. of the mixture composed of a part of the 

 medulla in water was injected, after which the wound was closed 

 with sutures and treated with antiseptics. It healed at once by first 

 intention. 



This rabbit remained well until the morning of July fourteenth 

 when he refused to eat. On the fifteenth he became quiet and was 

 not disposed to move about the cage. On the sixteenth symptoms 

 of paralysis were prominent. On the morning of July seventeenth, 

 he was found dead. 

 4 



