24 



The reacting division, as I have l)efore mentioned, was inoculated 

 every spring ; but this operation had nothing to do with the real 

 plan of experiment. It was done for purely scientific reasons. All, 

 or nearly all, cattle which were removed from the herd including 

 overtime and aborting cows, young and old animals, those not well 

 developed, etc., were butchered at the Copenhagen slaughter-house, 

 and carefully examined by me or in a few cases by my assistant. 



The result of the experiment appears from the following figures : 



April . . . 

 October 

 May . . . 

 October 

 April. . . 

 October 

 May . . . 

 October 



(1). One calf had insignificant inborn tuberculosis, one cow had 

 a few calcified knots in one, mediastinal gland. 



(2). One three year old cow had shown reaction even in May : 

 she was butchered, but I found no definite tuberculosis, only two 

 knots of the size of a pin head in which tuljerculous bacilli could not 

 be detected. The diagnosis therefore remains doubtful. 



The first year's result was thus unfavorable, for about 10 per cent 

 of the animals of the healthy division reacted on the repeating of the 

 test. This may be due partly to lack of care in making the division 

 the first year.* 



But 1 l)elieve that the chief cause was unfavorable local condi- 

 tions. In order to enter the healthy division it was necessary to go 

 over a walk over which the dung of the reacting division was carried 

 out. At the time this walk was very poorly paved and therefore 

 hard to clean. Infected particles could therefore very easily attach 

 themselves to the foot-gear of passing persons, and thus be intro- 

 duced into the healthy division. This walk was later radically 

 improved. 



Since October 1893 a result has been obtained which left nothing 



*At the first division of a tuberculous herd it must always be borne in mind 

 that single animals may have acquired germs so shortly before the test that they 

 are not yet in condition to show the infection by a reaction. 



