iv CURDLING FERMENT 21 



Prepare Cultures of Bacillus Anthracis 



From the agar-agar culture supplied inoculate 



(1) a potato tube; 



(2) a broth tube ; 



(3) an agar-agar tube (streak) ; 



(4) a gelatine tube (stab). 



Place (1) (2) and (3) in the warm incubator, and (4) in 

 the cool incubator. 



Examine after forty-eight hours : observe the charac- 

 teristic growths in the broth and gelatine. The 

 latter is slowly liquefied. 



Curdling Ferment 



Some micro-organisms will coagulate milk, others will 

 not. 



Inoculate four milk tubes with 



(1) Bacillus fluorescens ; 



(2) Bacterium coli commune ; 



(3) Bacillus anthracis ; 



(4) Bacillus prodigiosus. 



Place the inoculated tubes in the warm incubator. 



Examine the tubes after forty-eight hours : the milk 

 in (1) and (2) will be coagulated, that in (3) and 

 (4) will not 



