LESSON V 



Bacillus Anthracis Mode of Growth on Various Media Asporogenous 

 Cultures Acid Formation by Virulent Anthrax Bacilli Methods 

 of Attenuation Impressions (Sloped Gelatine) Methods of 

 Staining. 



Bacillus Anthracis 



(1) EXAMINE the tubes inoculated with Bacillus anthracis 

 and note the mode of growth on the various media. 



(2) Prepare a streak culture of Bacillus anthracis on 

 neutral litmus agar-agar, and place it in the warm 

 incubator. 



Examine it after forty-eight hours, and notice the 

 change in the colour of the litmus. The colour 

 gradually assumes a reddish tint on account of 

 the acid produced by the bacillus. 



(3) Prepare two hanging drop cultures of Bacillus 

 anthracis (vide p. 14), and place them in the warm 

 incubator. 



(4) Inoculate a broth tube containing carbolic acid 

 (1 : 1000), and keep it at 38 C. 



Examine it microscopically a week later : no spores 

 will be found (asporogenous growth). 



