CONTENTS xi 



PAGE 



ACID FOKMATION BY VlEULENT AND ATTENUATED BACILLI . 23 



METHODS OF ATTENUATION ... ... 23 

 STAINING OF THE BACILLUS ANTHRACIS 



(a) Loffler's Methylene-Blue ...... 23 



(b) Gentian-Violet 23 



(c) Gram's Method 24 



LESSON VI 



BACILLUS ANTHRACIS (continued) 



Hanging Drop Cultures (Staining) , . e 25 



Asporogenous Cultures (Staining) ..... 25 



Impression Specimens 26 



Examination of Fresh Tissues by Means of Films 

 Spleen of Mouse ... t 27 5 28 



LESSON VII 



BACILLUS ANTHRACIS (continued) 



Plate Culture Method 29, 30 



Staining of Spores , 30-32 



Ehrlich's, Loffler's, or Ziehl's Fuchsine .... 30-32 



SEPARATION OF HAY BACILLUS OUT OF HAY INFUSION . 33 



LESSON VIII 



BACILLUS ANTHRACIS (concluded) 



Impression Specimens (Gelatine Plates) .... 34 

 STAINING OF TISSUES IN FROZEN SECTIONS 



(a) Loffler's Methylene-Blue 35 



(b) Methylene-Blue and Eosine . . . . 35, 36 



(c) Methylene-Blue and Picrocarmine ... 36 



(d) Gram's Method . . . - . . . 36, 37 



