CONTENTS 



LESSON III 



PAGE 



STAINING OF MICRO-ORGANISMS (continued) 



Loffler's Methylene-Blue . . . .' . . 12, 13 



EXAMINATION OF MOULDS 



Aspergillus niger 13, 14 



HANGING DROP CULTURES 



(a) Streptococcus pyogenes . . . . . . 14 



(b) Bacillus filamentosus 14 



EXAMINATION OF BACTERIA IN A HANGING DROP 



Motility of Micro-organisms 15, 16 



LESSON IV 



STAINING OF HANGING DROP CULTURES 



(a) Loffler's Methylene-Blue . 17 



(b) Gram's Method 17-19 



GRAM'S METHOD 17-19 



STAINING OF Pus 



(a) Loffler's Methylene-Blue 20 



(b) Gram's Method 20 



INOCULATIONS WITH THE BACILLUS ANTHRACIS 



Potato ; Broth ; Agar-Agar ; Gelatine . . . . 21 

 CURDLING FERMENT 21 



LESSON V 



BACILLUS ANTHRACIS (continued) 



Mode of Growth on Various Media 22 



Hanging Drop Cultures 22 



ASPOROGENOUS CULTURES 



(a) Carbolic Acid Broth 22 



(b) Heat 23 



