LESSON VI 



Bacillus Anthracis (continued) Hanging .Drop Cultures Methods of 

 Staining Impressions Examination of Fresh Tissues by means 

 of Cover -glass Films Staining of Leucocytes for Eosinophile 

 Granules. 



Bacillus Anthracis (continued) 



(1) EXAMINE the litmus agar-agar tube inoculated with 

 Bacillus anthracis, and note the gradual disappearance of 

 the blue or purple colour. 



(2) Examine the hanging drop cultures with a high 

 power. 



Make a drawing. 



Stain (a) one of them with Loffler's methylene-blue in 

 the usual manner (vide p. 13) ; (b) the other according to 

 Gram's method. 



(3) Prepare a cover -glass specimen of the culture in 

 carbolic acid broth and stain it with Loffler's methylene- 

 blue, or according to Gram's method. 



Note the absence of spores. 



(4) Inoculate a litmus agar-agar tube from the broth 

 culture kept at 42 -5 C., and another litmus agar-agar tube 

 from an ordinary broth culture grown at 38 C. 



