vii SEPARATION OF THE HAY BACILLUS 33 



Separation of the Hay Bacillus (Bacillus subtilis) 

 out of Hay Infusion. 



Six tubes containing hay infusion are supplied. 



Heat them for five, ten, fifteen, twenty, twenty-five and 

 thirty minutes respectively in water at 80 C. 



From each tube inoculate a broth tube with three 

 platinum loops of the infusion and label them. 



Keep the six tubes in the cool incubator and examine 

 them on the next occasion. 



Pure cultures of the hay bacillus will probably be 

 obtained. 



