38 GENERAL BACTERIOLOGY LESSON 



Place the gelatine tubes in the cool incubator at 22 C., 

 the others in the warm incubator at 38*5 C. 



Staining of Flagella (Van Ermengem) 



Prepare the following solutions : 



(a) Osmic Acid (2 per cent solution) 1 part. 

 Tannin (10 to 25 per cent solution) 2 parts. 



To each 100 cc. of the tannin solution add four or 

 five drops of glacial acetic acid. 



(/3) Nitrate of silver (-25 to -5 per cent solution). 



(<y) Gallic Acid ... 5 grammes. 

 Tannin ... 3 grammes. 

 Fused acetate of soda . 10 grammes. 

 Distilled water . .350 cc. 



Boil the cover-slips to be used in the following solution : 



Potassium bichromate . 60 grammes. 



Concentrated Sulphuric Acid 60 grammes. 

 Water . . . .1000 cc. 



Then wash them repeatedly in water. Keep them in 

 absolute alcohol and before use allow them to dry, without 

 wiping, by placing them in a vertical position, protected 

 from dust. 



Bacillus of typhoid fever and Vibrio cholerce Asiatics. 



Carefully suspend one or two loops of an agar-agar culture 

 (ten to eighteen hours old) in a watch-glassful of distilled 

 water. 



