LESSON IX 



Examination of Vibriones Choleras Asiatics, Finkler - Prior, and 

 Metchnicovi in Cultures and in the Animal Body Plate Cultures 

 Germicidal Action of Sunlight Hanging Drops. 



Cholera (continued) 



(1) EXAMINE and compare the tubes inoculated with 

 Vibrio cholerse Asiaticse, Vibrio Finkler-Prior, and Vibrio 

 Metchnicovi respectively. 



(2) Prepare cover -glass specimens of Vibrio cholerse 

 Asiatics, Vibrio Finkler-Prior, and Vibrio Metchnicovi in 

 the usual manner. 



Stain them with dilute aniline gentian- violet or dilute 

 carbol fuchsine, as follows : 



(a) Filter -some of the staining solution into a watch- 

 glass and dilute it with alcohol (equal parts). 



(b) Stain for about three minutes, previously clearing 

 the specimen with acetic acid, if necessary. 



(c) Wash in water, dry and mount. 



Examine with ^ in. oil immersion and compare the 

 three forms. 



