LESSON ix EXAMINATION OF VIBRIOS 41 



(3) Prepare cover-glass specimens of Spirillum rubrum 

 (gelatine stab culture). 



(a) Melt the gelatine tube in the warm incubator. 



(b) Prepare films and pass them three times through the 

 flame in the usual manner. 



(c) Wash the films in warm water for two to five 

 minutes, in order to remove the gelatine. 



(d) Stain in dilute aniline gentian - violet or carbol 

 fuchsine, as above. 



(e) Wash in water, dry and mount. 

 Examine with ^ in. oil immersion. 



(4) From a liquefied gelatine culture of Spirillum 

 rubrum prepare a gelatine stab culture and keep it at 



22 C. 



(5) Prepare hanging drop cultures of Vibrio cholerse 

 Asiatics, using 1 per cent peptone solution. 



Place them in the warm incubator. 



(6) Examine the peritoneal fluid of the guinea-pig supplied 

 for Vibrio cholerce Asiaticce. 



(a) Open the abdominal cavity of the animal in the usual 

 manner (vide p. 27). 



(b) Make a capillary pipette, and draw up some of the 

 peritoneal fluid into its bulb. 



(c) Prepare films from the fluid removed, in the usual 

 manner, and pass them three times through the flame. 



(d) Clear them with acetic acid and then stain with 

 aniline gentian-violet, as already described (vide supra). 



Examine with i n - il immersion. 



