42 GENERAL BACTERIOLOGY LESSON 



(e) Other films should be stained with eosine and methy- 

 lene-blue, without previously clearing them with acetic 

 acid (vide p. 28). 



Examine with -^ in. oil immersion : note the eosino- 

 phile granules in the leucocytes. 



Plate Cultures of Vibrio Cholerae Asiaticse 



Open the thorax (of a guinea-pig inoculated intra- 

 peritoneally with the Vibrio cholerae Asiaticae) in the 

 following manner : 



(a) Moisten the skin with spirit. 



(b) Eeflect it freely on both sides. 



(c) Cauterise the exposed surface, and with a pair of 

 sterilised scissors and forceps remove the sternum. 



(d) Now cauterise with a heated glass rod a spot on the 

 surface of the heart. 



(e) Thrust a sterile capillary pipette, the ends of which 

 have been broken off, through the heart's wall into an 

 auricle or ventricle and remove a trace of blood by suction, 

 and fuse the end to which suction has been applied. 



(/) Loosen the cotton-wool plug of a liquefied gelatine 

 tube and insert the pipette into the test-tube by carefully 

 pushing it through the cotton-wool. 



(g) Push the pipette into the lumen of the tube, till it 

 almost touches the gelatine. 



(h) Apply very gentle heat to the bulb, till a drop of 

 blood falls into the gelatine. 



(i) Withdraw the pipette and shake the inoculated tube. 



