46 GENERAL BACTERIOLOGY LESSON x 



(g) Pour a little aniline oil over the specimen, which is 

 now quite black, and by alternately tilting first one and 

 then the other end of the slide, allow the oil to flow to 

 and fro over the section. The aniline oil will take up the 

 gentian-violet greedily, and gradually become saturated. 

 When this has taken place, remove the oil with blotting- 

 paper and pour fresh oil over the specimen. Repeat this 

 process till no more stain comes away. 



(Ti) Eemove the oil with blotting-paper, and wash the 

 specimen thoroughly with xylol, which must be frequently 

 changed. 



(i) Mount in xylol balsam and examine with low and 

 high powers. 



It is essential that the whole process be completely 

 carried through with the section on the slide. 



Sections of Mycetoma (Madura disease) may be 

 stained in the same manner. 



