LESSON XI 



Pyogenic Cocci Cultures Hanging Drops Staining of Pus preserved 

 in Carbolic Acid Staining of Fresh Pus Staining of Gonorrhceal 

 Pus. 



Pyogenic Cocci 

 (1) MAKE a broth culture of the 



(a) Streptococcus pyogenes ; 



(b) Streptococcus pneumonise ; 



(c) Streptococcus erysipelatos. 



Keep them at 38*5 C., and compare them from day 

 to day. 



(2) Make also agar-agar streak cultures of the same 

 organisms. 



Keep them at the same temperature, and compare 

 them from day to day. 



(3) Make gelatine stab cultures of the same organisms 

 and keep them at 22 C. 



They all grow slowly in gelatine. 

 Compare them from day to day. 



(4) Prepare agar-agar streak cultures of the Staphylo- 

 coccus pyogenes albus, aureus, and cereus flavus respec- 

 tively, and keep them at 38*5 C. 



