xi STAINING OF PUS 49 



(/) Dry them between blotting-paper and again pass 

 them three times through the flame. 



(g) Now stain them in the usual manner with dilute 

 aniline gentian- violet, or by Gram's method. 



Mount in xylol balsam and examine with ^- in. oil 

 immersion. 



(9) Prepare specimens of the fresh pus supplied which 

 contains Staphylococci. 



Prepare thin films in the usual manner, clear them in 

 acetic acid, and stain with eosine and methylene-blue in 

 the following way (vide p. 28) : 



(a) Stain with eosine. 



(b) Wash, and dry with filter-paper. 



(c) Heat again and stain with Loffler's methylene-blue. 



(d) Wash, dry and mount. 

 Examine with -^ in. oil immersion. 



(10) In the same manner prepare and stain specimens of 

 gonorrhoeal pus. (Eosine and methylene-blue.) 



Examine with ^ i n - ^ immersion : note the cocci 

 in the cells. 



