52 GENERAL BACTERIOLOGY LESSON xn 



(b) Eemove the superfluous water with a piece of filter- 

 paper. 



(c) Filter a little aniline gentian-violet on to the section, 

 and allow it to act for twenty to thirty minutes. 



(d) Eemove the aniline gentian-violet with blotting- 

 paper, and wash with '6 per cent saline solution. 



(e) Pour a few drops of the following iodine solution on 

 to the section : 



Iodine .... 1 gramme 



Potassium iodide . . 2 grammes 



Water . . . . 100 cc. 

 Allow this to act for two to three minutes. 



(/) Remove the iodine solution with blotting-paper, 

 and decolourise with a solution of aniline oil (2 parts) and 

 xylol (1 part), in exactly the same manner as described in 

 the case of actinomycosis (vide p. 46). 



(g) Wash thoroughly with xylol to remove all trace of 

 the aniline oil. 



(h) Mount in xylol balsam and examine with a high 

 power. 



The fibrin network and the Pneumococci will be 

 distinctly shown in most cases. 



To make the specimen more effective stain the section 

 previously with picrocarmine. 



