xiii DIPHTHERIA AND LEPROSY 57 



Keep the first two at 38 -5 C. and the gelatine tube at 



22 C. 



(2) Prepare cover-glass specimens from an agar-agar 

 culture and stain with aniline gentian-violet. 



(a) Leave them in the stain for five to ten minutes. 

 (5) Wash thoroughly in water. 

 (c) Dry and mount. 



Examine with -^ in. oil immersion : note the clubbed 

 forms. 



(3) Diphtheritic membrane. 



Stain sections of diphtheritic membrane with the 

 eosine methylene-blue mixture in the usual manner (vide 

 p. 35). 



Examine with low and high powers. 



(4) Prepare hanging drop cultures in the usual manner 

 and keep them at 38 -5 C. 



Examine them from day to day, so as to study the 

 peculiar forms which the diphtheria bacillus often 

 shows. 



Leprosy Tissue 



(1) Stain with picrocarmine, and then according to 

 Weigert's fibrin method (vide p. 51). 



Examine with low and high powers and ^ in. oil 

 immersion, and note the arrangement of the 

 bacilli. 



(2) Stain a section with a filtered carbol fuchsine solution. 



