LESSON XIV 



Tubercle Bacillus Staining of Fresh Sputum Staining of Carbolised 

 Sputum Staining of Frozen Sections Staining of Paraffin Sections. 



(1) EXAMINE the cultures made on the last occasion. 



(2) Prepare films of the recent cultures of the Bacillus 

 typhosus and the Bacterium coli commune and stain them 

 with methylene-blue. 



(3) Prepare films of the fresh culture of the diphtheria 

 bacillus, and stain them with aniline gentian-violet. 



Tubercle Bacillus 



(1) Staining of fresh sputum. 



(a) Pour out the sputum in a thin layer into a glass dish, 

 and placing the latter on a dark background select one of 

 the characteristic yellowish particles, and pick it out with a 

 pair of fine-pointed forceps. 



(b) Prepare thin films by squeezing the suspected matter 

 between two clean cover-glasses. 



(c) Allow them to dry in the air, and then pass them 

 three times through the flame. 



