xiv STAINING OF TUBERCULOUS TISSUES 63 



(e) Wash it in 70 per cent spirit, till no more stain 

 comes away. 



(/) Again wash the section in distilled water, or '6 per 

 cent saline solution. 



(g) Counterstain it, for two minutes in Loffler's methylene- 

 blue if carbol fuchsine was used, or in vesuvine for five 

 minutes if carbol gentian-violet was employed. 



(h) Wash it thoroughly in distilled water. 



(i) Dehydrate it in absolute alcohol and clear it in 

 xylol. 



(k) Transfer the section on to a slide and mount it in 

 xylol balsam. 



Examine it with a high power and T ^ in. oil 

 immersion. 



(ii.) Slow Method. 



(a) Place a section in aniline fuchsine or aniline gentian- 

 violet for twelve to twenty-four hours. 



(b) Wash it rapidly in water and decolourise it in 25 per 

 cent hydrochloric acid, and then proceed as above. 



This method gives extremely good results, 

 (iii.) Gram's Method. 



(a) Stain a few sections first with picrocarmine in the 

 ordinary manner (vide p. 36). 



(b) Place them in aniline gentian-violet for twenty-four 

 hours. 



(c) Wash a section rapidly in water. 



