LESSON XV 



Tubercle Bacillus (concluded] Films of Pure Cultures Avian Tuber- 

 culosis Glanders Tetanus Bacillus Paraffin Sections of Ac- 

 tinomycosis Pyaemia Phagocytosis in the Frog Inoculation 

 of the Pithed Frog Hanging Drops of Frog's Lymph Effect of 

 Temperature on Phagocytosis. 



Tubercle Bacilli (pure cultures) 



(1) Mammalian Tubercle. 



(a) Prepare cover-glass films from the emulsion supplied. 



(b) Stain them with warm carbol fuchsine or carbol 

 gentian- violet, decolourise with 25 per cent hydrochloric 

 acid and 70 per cent alcohol, as in the case of tubercular 

 sputum. Counterstaining is, of course, unnecessary, since 

 no other organisms are present. 



(2) Avian Tubercle. 



Prepare cover-glass films from the emulsion supplied, 

 and proceed as above. 



Examine with ^ in. oil immersion and compare the 

 two kinds of bacilli. 



