68 GENERAL BACTERIOLOGY LESSON 



Glanders Horse's Lung (paraffin sections) 



(i.) Slow Method. 



(a) Fix the sections on clean cover-glasses and dissolve 

 out the paraffin (vide p. 64). 



(b) Place them in Loffler's methylene-blue for six to eight 

 hours. 



(c) Wash them in distilled water or in *6 per cent saline 

 solution. 



(d) Then place them in a solution of tannic acid (1 in 

 10) for four to five hours. 



(e) Wash thoroughly in water. 

 (/) Dehydrate in absolute alcohol. 

 (g) Clear in xylol and mount. 



Examine with a high power and ^ in. oil immersion. 



(ii.) Quick Method. 



(a) Fix the sections on clean cover -glasses and dissolve 

 out the paraffin as before. 



(b) Float the sections for ten to thirty seconds on a 

 solution of carbol methylene-blue (i.e. a saturated solution 

 of methylene-blue in 5 per cent carbolic acid, which must 

 always be filtered before use). 



(c) Then wash the sections in water. 



(d) Wash them in a 10 per cent solution of tannic acid 

 for half a minute to one minute. 



(e) Counterstain in a weak solution of eosine or acid 

 fuchsine in 10 per cent tannic acid, till the sections are red. 



