xv STAINING OF TETANUS BACILLI 69 



(/) Now wash them in water till they are pink. 



(g) Dehydrate in absolute alcohol, clear in xylol and 

 mount. 



Examine with a high power and -^ in. oil immersion. 

 The glanders bacilli are blue on a pink back- 

 ground. 



Tetanus 

 Broth Culture. 



(a) Prepare cover-glass films in the usual manner, and 

 pass them three times through the flame. 



(b) Clear them in acetic acid, wash and dry, and pass 

 them again through the flame. 



(1) Stain some of the films with Loffler's methylene-blue 

 or gentian- violet for two to three minutes. 



Examine with -^ in. oil immersion. Observe the 

 drum-stick shape of the spore-bearing bacilli. 



(2) Stain other cover-glass films for spores with Loffler's 

 fuchsine and methylene-blue (vide p. 31). 



Examine with -^ in. oil immersion. The terminal 

 spores are stained red. 



Paraffin Sections of Actinomycosis Hominis 



(1) WeigerVs fibrin method. 



(a) Fix the sections on clean cover-glasses and dissolve 

 out the paraffin (vide p. 64). 



(b) Stain with picrocarmine for half an hour. 



