76 GENERAL BACTERIOLOGY LESSON 



Then examine its lymph again by means of hanging 

 drops and stained films. 



Note the disappearance of the anthrax bacilli, the 

 well-marked phagocytosis and "eosinophile leuco- 

 cytosis." 



(2) Ancesthetised frog. 



A frog has been anaesthetised (chloroform-ether mixture), 

 and during the narcosis inoculated under the skin of the 

 right thigh with a virulent culture of anthrax bacilli ; and 

 it has then been kept at the ordinary temperature in a moist 

 chamber. 



Twenty-four hours later it has been pithed. 



Now prepare hanging drops and stained films of its 

 lymph. 



Examine them with a high power and -^ in. oil 

 immersion. 



Note that the anthrax bacilli have grown well, and 

 that there is no phagocytosis or "eosinophile 

 leucocytosis." 



This proves that the immunity of the frog from anthrax 

 does not depend merely on the body temperature. 



(3) Phagocytosis in the hanging drop. 



(a) Prepare a moist chamber, as described on p. 15. 



(b) Pith a healthy frog, and withdraw some of its lymph 

 or peritoneal fluid in the usual manner. 



(c) Place a drop of the lymph or fluid on the centre of a 

 sterile cover-glass, avoiding air-bubbles. 



