112 BACTERIOLOGICAL ANALYSIS LESSON vn 



Aspirate for one or two hours, counting the number 

 of aspirations performed, so as to ascertain the 

 volume of air taken and the rapidity of aspiration. 



(8) Now remove the sugar tube and shake out the 

 sugar into a flask containing 50 cc. of broth, which of 

 course must be sterile. 



(9) Liquefy several gelatine tubes, and add to each from 

 1 to *5 cc. of the sugar solution, and pour plates in the 

 usual manner ; and work them up as before. 



Count the colonies in the plates in the way described on 

 p. 95. 



