LESSON VIII 



Examination of Air and Dust (concluded) Anaerobic Germs Examin- 

 ation of Soil Surface Soil Tetanus and Malignant (Edema 

 Bacilli Anaerobic Growth in an Exhausted Flask Anaerobic 

 Growth in Hydrogen Fractional Separation of Tetanus Bacilli. 



Examination of Air and Dust (concluded) 

 IV. ANAEROBIC GERMS IN AIR AND DUST. 



(1) Put up an apparatus as shown in the diagram 

 (Fig- 10). 



(2) Place the Wolff bottle 



(A) in the water bath, which A 



must be kept steadily at Aspirator 

 38*5 C. 



(3) Eemove the cotton- 

 wool plug from a, and by 



f , -, . , , A, Sterilised Wolff bottle containing 50 



means of the aspirator slowly to 100 cc . of sterile liquid grape . 

 suck air through the warm 

 gelatine for half an hour to 

 one hour. 



(4) Now fuse off the tube at /', and aspirate again 

 until the flask (A), which must be kept in the water bath 

 all the time, is exhausted. 



I 



PIG. 10. 



sugar gelatine ; B, water bath kept at 

 38 '5 C. ; a and &, tubes plugged with 

 cotton-wool ; C, clamp on tube con- 

 necting apparatus with aspirator. 



