LESSON XI 



Examination of an Animal dead of a Bacterial Disease Anthrax 

 Pyocyaneus Septicaemia Cholera Asiatica Rapid Method of 

 Embedding Tissues in Paraffin. 



How to Examine an Animal dead of Bacterial Disease 



Three animals are supplied : 



(a) mouse inoculated with Bacillus anthracis ; 



(b) guinea-pig inoculated intraperitoneally with 



Bacillus pyocyaneus ; 



(c) guinea pig - inoculated intraperitoneally with 



Vibrio choleras Asiatics. 



(1) Nail the animal out on a wooden board, thoroughly 

 washed with mercuric chloride 1 : 1000. 



(2) Moisten the hairy surface of the animal's abdomen 

 with spirit, and then wash it with mercuric chloride 1 : 1000. 



(3) With a pair of sterilised forceps and a pair of sterilised 

 scissors carefully reflect the skin from the abdomen and 

 chest. 



(4) Thoroughly cauterise the exposed surface with a red- 

 hot glass rod, and then open the chest and abdominal cavity 

 with sterile instruments. 



