LESSON VIII 



Ferments and Enzymes Action of Chloroform on Ferments and 

 Enzymes Action of Heat on Enzymes Separation of Enzymes 

 (Alcohol Precipitation). 



Ferments and Enzymes 

 A. FERMENT AND ENZYME IN YEAST. 



(1) Prepare a dilute solution of cane sugar. 



(2) Test it with Fehling's solution : no reduction of 

 copper. 



(3) Pour a little of this sugar solution into two test-tubes, 

 and add a little yeast. 



(4) Keep both tubes at 38 -5 C. 



(5) After a few hours (four to six hours) test one tube 

 with Fehling's solution : marked reduction of copper. 



Invertine splits up cane sugar into dextrose and 

 Isevulose. 



(6) Test the other tube next day with Fehling's solution : 

 no reduction of copper. 



Alcohol fermentation has taken place. 

 M 



