LESSON X 



Ptomaines Cadaverine Putrescine 



Ptomaines 

 CADAVERINE AND PUTRESCINE (Udransky and Baumann). 



(1) Allow a solution of white of egg to putrefy for 

 several days. 



(2) Distil to a small volume : indol, scatol, and phenol 

 pass over in the distillate, and are disregarded. 



(3) Filter the residue. 



(4) To the filtrate add an equal volume of a 10 per 

 cent solution of caustic soda. 



(5) Shake, and gradually add, drop by drop, a solution of 

 benzoyl chloride : a crystalline precipitate appears. 



(6) Allow to stand for several days. 



(7) Filter. 



(a) Strongly acidulate the turbid (a) The residue must be digested 

 nitrate with sulphuric acid (benzoic in spirit. 



acid passes off). 



(b) Shake up with three times (b) Filter and evaporate the 

 its volume of ether and nitrate to a small bulk. 



