THERMAL DEATH-POINTS 79 

and a dry layer swarming with spores of the Bacilli 
was left, the slips were put away in a cabinet, pro- 
tected from dust, and there left for nearly six years. 
Then, in 1883, the dry layer was at different 
times scraped off from the glass slips, the fragments 
were put into a purified test-tube, with about one 
drachm of distilled water, and were well shaken, so 
as to break up and distribute the particles and form 
a kind of emulsion. Here then we had an inoculat- 
ing material, undoubtedly full of “old hay germs” 
which had undergone a prolonged desiccation, and 
were, consequently, in the condition deemed most 
favourable for enabling them to escape the destructive 
influence of heat. 
Two sets of experiments were made with this 
mixture ; one in which this inoculating material was 
allowed to soak in the distilled water for four hours at 
a temperature of 112° F., so as to imitate as much 
as possible what would happen in the preparation of 
an infusion; and another in which there was no 
preliminary soaking of the inoculating material for 
more than a very short time prior to the process of 
heating to which it was submitted. Sterilised acid 
urine was again used as the nourishing fluid in each 
set of experiments. 
(1) Laperiments with the Spore Liquid previously 
marntained at 112 Lf. for four hours.—At the 
expiration of the four hours, the spore liquid was 
added to the sterilised urine contained in a sterilised 
receiver, as before, in the proportion of two minims 
to the ounce, 
