7 
Twelve two-ounce retorts were then charged with 
the inoculated fluid, and the necks of the retorts 
were drawn out in the blow-pipe flame. Each fluid 
was boiled about one minute overa flame and sealed 
during ebullition ; after which the retorts were in-| 
verted and finned into a can of boiling water, 
for different periods :— 
80 THE EVOLUTION OF LIFE> 
2 were boiled in the can for 5 minutes. 
2 is . ter te 
2 5 7: Pas es 
2 y : 20 ws. 
7 Dy 25 9 
” yy 30 0) 
NO 
The twelve retorts, after being heated, were 
placed in the incubator at 120° F., and the results — 
were as follows :—The fluid in one of the retorts 
which had been heated for 30’ to 212° F., became 
turbid in two days, and was found to swarm with 
Bacilli ; while those in the other 11 retorts remained 
quite clear at the expiration of 14 days. 
The scum containing Bacillus spores was scraped 
from another slip, and after soaking in a test-tube 
containing a drachm of distilled water for four hours 
at 212 F., as before, it was dealt with in a rather 
different manner. 
This spore liquid was added, in the same pro- 
portion, to a stock of sterilised urine contained in a 
purified Lister’s receiver. Meanwhile a number of 
two-ounce sterilised cork flasks had been prepared, 
which, with the aid of an assistant, were charged 
rapidly, and in the most careful manner, after the 
