


~ 2 >i 
ah 
THERMAL DEATH-POINTS 81 
fluid had been boiled for different periods. After 
charging, the flasks were quickly recorked, and 
placed in the incubator at 120° F. This trial 
differed from the other, therefore, in the fact that 
the flasks contained air. It led to the following 
results :— 
Results. 
; , ( 2 turbid in 24 hours. 
3 flasks charged after fluid boiled 5 { pciese tees dave 
3 9 ”? 39 ) 
ay { 2 turbid in 24 hours. 
7 r clear after 5 days. 
18 other flasks were charged with 
this fluid, which had been boiled 
ro’; and then the fluid in each 
flask was boiled for 5’ more, 
making a total boiling for each 
of 45". 
All the 18 fluids 
> quite clear after 5 
days.! 

J 
Thus, in these 36 experiments only 4 flasks 
showed any evidence that the Bacilli spores, after 
prolonged desiccation, were able to survive a boiling 
of 10’, and only 1 that they might be able to resist 
boiling for 30’. 
(2) Experiments with Spore Liquid heated without 
such a prolonged preliminary soaking.—Six two- 
ounce retorts were charged, more than half full 
with sterilised urine, inoculated as: before, and in 
the same proportion, with the scrapings, swarm- 
ing with Bacillus spores, from another glass slip. 
The necks of the vessels were drawn out, as before, 
1 It was found useless to keep these inoculated fluids under obser- 
vation for a longer period. Growth and multiplication occurred within 
three days, if at all. 
F 
