
EXAMINATION OF PASTEUR’S DOCTRINES _ 105 
of Bacteria and Leptothrix, embedded in a hyaline 
matrix. There were also Bacteria in the liquid. 
Tuirp Sertes—( Dec. 27th.) 
It appeared to me desirable to ascertain whether 
the condition of the internal surface of the glass 
vessels exercised any influence on the result. | 
therefore heated two retorts to 250 C., keeping 
them at that temperature for half an hour, and closed 
them while hot in the blow-pipe flame. These Dr 
Bastian charged by breaking off their points under 
the surface of a neutral infusion of turnip with 
cheese, freshly prepared for the purpose, without 
employing any of the rind. The retorts were 
boiled and sealed in the same way as before, except- 
ing that whereas one was boiled only five minutes 
the other was boiled ten minutes. The specific 
gravity of the infusion used was 1013. A third 
uncalcined retort was charged with some of the same 
infusion containing no cheese. ‘This was also 
boiled for ten minutes. 
I was out of town from the 28th to the 30th, and 
therefore did not examine the retorts until the 31st. 
Dr Bastian informed me that on the 28th, twenty- 
one hours after preparation, the liquids in both the 
calcined retorts were distinctly turbid, the temperature 
of the water bath being 32° C.: and that sixty-six 
hours after preparation, whilst the turbidity was 
much more marked, each flask also contained what 
appeared to be a “pellicle,” which had formed and 
sunk. At this period the fluid in the third flask had 
also become very decidedly turbid. 
