
IMPORTANT NEW EXPERIMENTS 135 
by heating it ina flame before immersing its open 
capillary extremity in the requisite quantity of liquor 
potassze, contained in a minute porcelain capsule. 
When the whole of the measured amount of alkali 
had thus been forced into the glass tube, this was 
inverted, and its capillary extremity was sealed in 
the spirit-lamp flame. Its neck was then wrapped 
round with cotton-wool, and the tube itself was 
inserted into one of the flasks in such a manner that 
the cotton-wool might act as a plug thereto, while 
the capillary extremity of the tube just touched the 
bottom of the vessel. 
The flasks being thus charged and arranged, the 
urine in its altered acid state was boiled over a 
flame for five minutes. When the fluid had cooled, 
the tube was pressed down slightly so as to break 
off its capillary extremity ; and immediately after- 
wards a flame was applied to the external bulb of 
the tube, so as to expand its contained air. The 
measured amount of liquor potassee was thus ex- 
pelled into the sterilised urine; and the flask was 
then placed in an incubator and maintained at a 
temperature of 104°-113° F. (40°-45° C.).} 
Some tentative experiments were made in this 
manner with fresh urine, whose specific gravity 
varied from 1020 to 1025, and whose acidity was 
such that 7-15 minims of liquor potassee per ounce 
were required for neutralisation. In nearly every 
case it was found that the urine became lighter- 
coloured and turbid in two or three days. Other 
1 No higher incubating-temperatures were employed in these par- 
ticular experiments. 
