OTHER NEW EXPERIMENTS 173 

additional diminution of acidity was also equivalent 
to m. id of liquor potasseze. 
“Other experiments were made to ascertain 
whether the change of urea into carbonate of 
ammonia would still go on at lower incubating 
temperatures ; and this time the urine was one of 
much higher acidity and specific gravity. Exactly 
one fluid- ounce was used, as before, in each 
experiment. | 
Treatment, Results. 
1. Boiled for 2’ over flame,| 1. Acidity=m. xviij of liq. 
ye 
Urinewhoseaci- | 8 in can. pot. 
‘dity was exactly} 2. Boiled for 2’ over flame,} 2. Acidity=m. xvj of liq. 
neutralised by Sin: can. Left at. 33°F.) pot. 
m. xxj of liquor \ for 7 days. 

potasse to the/ 3. Boiled for 2’ over flame,| 3. Acidity= iij of li 
: ; a a y=m. xiij of liq. 
fluid-ounce. S incans. Lelt: at: 122’ F. pot. 
or 7 days. 
“From this it appears that the diminution of acidity 
goes on ata very appreciable rate, even at the compar- 
atively low temperature of 83° F.1. Yet M. Pasteur 
said (see p. 169) that no alteration in acidity would 
be found even after an exposure to eighteen months 
at that temperature!” 
We may turn now to the question of the exact 
mode in which M. Pasteur attempted to reproduce 
my experiment with boiled urine and super-heated 
liquor potassze. 
It will be noted from what M. Dumas said to me 
in his letter of May 5 (p. 159), that the Commission 
had allowed M. Pasteur to perform his experiments 
1 The rate of change is distinctly greater with the higher incubating 
temperature of 122° F., as I have shown by another table (/oc. czz., 
p- 40). 
